Accuracy And Sensitivity Is Attainable Using Elisa Test Kits

By Sally Delacruz


The new method in the field of diagnosis uses special tools that help find diseases and other foreign bodies in an easy way. This method involves an enzyme called immunoassay. Elisa test kits are also a key ingredient in this test. The whole test depends on color change as the main indicator of the existence or lack of existence of foreign bodies.

These kits are always made with the purpose of providing accuracy and consistency in research results. They are therefore required to be sensitive, easy to follow, precise and specific to individual tests. This can be easily achieved through rigorous testing. The testing may involve validating with many samples from, serum, saliva, feces and urine.

The main reason for manufacturing these kits is enhancement of consistency and accuracy by way of promoting visibility. Devices used in testing, therefore, have to be easy to understand and sensitive to changes in the reagents. They also have to be specific to certain tests and must also be precise. Rigorous testing is a very important exercise in ensuring that the kits satisfy all these requirements.

These tools are very important in accelerating research. A kit that cannot deliver the biological sensitivity and reproducibility required in studies that take a long time is not good for use. It is likely to cost the buyer a lot of his resources and time. Whoever wishes to buy these kits, therefore, needs to be very cautious since many companies promise many things only to disappoint researchers.

Besides antibodies, many other factors are crucial during the manufacture of these tools. For instance, rigorous antibody screening must be done, good standards chosen, steps for sample preparation developed and essays properly validated with the help of matrices. All these process are done with the purpose of attaining effectiveness.

The kits are capable of detecting items that are present in low concentrations for the sake of diagnosing and treating a number of diseases during their early stages. It has, previously been used in diagnosing different types of illnesses in both animals and plants. This technology has also been used for ensuring quality control in many industries.

Usually, researchers take antigens from samples and attach them onto surfaces. They then apply specified antibodies with the intention of binding them with antigens. Special food substances are then applied. The reaction that follows gives a signal in the form of color change.

In carrying out two tests at the same time using the same tools, the rods are usually put in a beaker containing samples. Chromogen is then used in washing and incubating them. The rods that make up the kit are composed of 12 Ogives that are always protruding. This process takes place when ogives are dipped in minute wells composed of micro-plates made up of reagents.

It is always practical to use various reagents with the equipment. Hence, detecting various antibodies and antigens is also a possibility. To do this, the Ogive has to be sensitized to the different reagents. Another way of improving the sensitivity of the devices is through increasing the amount of reagents used. The sensitivity used can be either environmental or clinical.

The fact that these facilities can be used in establishing the amount of antibodies in the human body has made it possible for the test to be used in HIV testing. Their portability is also a cause for their popularity in carrying out such tests. Nevertheless, caution should be taken in determining the best kits.




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