Technology in medicine has taken a new dimension. With regards to testing for presence of diseases and other anomalies, Elisa technology has taken over. The cardiac Elisa kits have been particularly so good. They are devices capable of working with the hearts of almost all animals in the world to establish any defects on it.
This process depends is an enzyme dependent process that uses color change as an indicator of reactions in reagents. The process works through an enzyme immunoassay which combines with antigens producing the subsequent color change. This test is capable of establishing the presence of both antibodies and antigens.
This exercise is capable of detecting antibodies and antigens in patients. This is very useful; it helps detect illnesses before they develop into chronic problems. Doctors are able to work on it during its early stages of development. The patient will, therefore, be able to eliminate the problems at an affordable value.
For proper working of these devices precision, sensitivity, accuracy and ability to work on a wide range of problems, and give many details are very important. Sensitivity helps in detecting any slight change in reaction when they are mixed with the samples. Accuracy, on the other hand, is required in ensuring that no errors are made in the experiments. The devices are also supposed to be specific to individual heart problems.
It is also important that the instruments are made in a way that makes them stable. To attain stability, one must cut down on the rate loss of these activities. This is possible through proper storage. Stability can also be achieved through minimizing the effects of the surrounding on the set-up. This means temperature, humidity and pressure have to agree with the standard lab requirements. There should be somebody to control incubator temperatures. If only one person is allowed to work on the research from beginning to end, it will be easy to achieve stability.
Before the experiment is done, the researcher must prepare all the standards, samples and reagents. Some samples are then added to each well and incubated for approximately two hours. Having done this, the researcher should then aspire the previous mixture before adding a small amount of the reagent. He/she must then incubate the mixture for one hour. The substances are once again aspired and washed three times before a solution of the substrate is added and then incubated for 20-25 minutes. Lastly, a stopping solution is added to end the reaction.
This process applies the sandwich enzyme principle. The plate that comes with the kit is coated with antibodies that are specific to the defect to be diagnosed. Standards/samples are later put on the plate as is appropriate. The samples or standards contain biotin-conjugate antibodies that are specific for the defect. Avidin conjugate is then added to every plate before incubating.
After the addition of substrate solutions, it is only the wells that will contain type 3 of Tropin I. The reagents will then show a change in color. The reaction is brought to an end by adding a solution of sulphuric acid, and the change in color is measured is a special style.
This process depends is an enzyme dependent process that uses color change as an indicator of reactions in reagents. The process works through an enzyme immunoassay which combines with antigens producing the subsequent color change. This test is capable of establishing the presence of both antibodies and antigens.
This exercise is capable of detecting antibodies and antigens in patients. This is very useful; it helps detect illnesses before they develop into chronic problems. Doctors are able to work on it during its early stages of development. The patient will, therefore, be able to eliminate the problems at an affordable value.
For proper working of these devices precision, sensitivity, accuracy and ability to work on a wide range of problems, and give many details are very important. Sensitivity helps in detecting any slight change in reaction when they are mixed with the samples. Accuracy, on the other hand, is required in ensuring that no errors are made in the experiments. The devices are also supposed to be specific to individual heart problems.
It is also important that the instruments are made in a way that makes them stable. To attain stability, one must cut down on the rate loss of these activities. This is possible through proper storage. Stability can also be achieved through minimizing the effects of the surrounding on the set-up. This means temperature, humidity and pressure have to agree with the standard lab requirements. There should be somebody to control incubator temperatures. If only one person is allowed to work on the research from beginning to end, it will be easy to achieve stability.
Before the experiment is done, the researcher must prepare all the standards, samples and reagents. Some samples are then added to each well and incubated for approximately two hours. Having done this, the researcher should then aspire the previous mixture before adding a small amount of the reagent. He/she must then incubate the mixture for one hour. The substances are once again aspired and washed three times before a solution of the substrate is added and then incubated for 20-25 minutes. Lastly, a stopping solution is added to end the reaction.
This process applies the sandwich enzyme principle. The plate that comes with the kit is coated with antibodies that are specific to the defect to be diagnosed. Standards/samples are later put on the plate as is appropriate. The samples or standards contain biotin-conjugate antibodies that are specific for the defect. Avidin conjugate is then added to every plate before incubating.
After the addition of substrate solutions, it is only the wells that will contain type 3 of Tropin I. The reagents will then show a change in color. The reaction is brought to an end by adding a solution of sulphuric acid, and the change in color is measured is a special style.
